Sarooj Nadeem

◦ Experience with IPSC/ESC cell line culture.
◦ Performed data generation, collection, documentation, analysis and presentation. ◦ Prepared variety of different written communications, reports and documents.

◦ Worked within applicable standards, policies, and regulatory guidelines to promote safe working environment. 

◦ Evaluated and optimized beta cell differentiation protocol using 2D/3D culture method.
◦ Designed and performed standard molecular biology assays.

◦ Analyze sequencing data – Sanger and NGS. Troubleshoot got data if necessary ◦ Performed Flow cytometry analysis on differentiated beta like cells from hIPSCs
◦ Conducted Confocal microscopic imaging on IPSCs
◦ Fluorescent Microscopy

◦ Flow cytometry
◦ ELISA, Cloning, q/PCR
◦ Western Blotting, plasmid purification, cloning, agarose and polyacrylamide gel electrophoresis and restriction

digests
◦ Sanger DNA Sequencing
◦ Performed data generation, collection, documentation, analysis and presentation.

Research project: Investigating the role of serotonergic regulation in diabetes type II and proliferation of hIPSCs derived pancreatic beta cell
Techniques Used:

Maintaining Stem cell culture, Gene cloning, Confocal microscopy, Flow cytometry, ICC/IHC/IFC

Cloning/plasmid construction and qPCR

Organizing lab meetings

Maintaining cell lines

MASTER'S THESIS RESEARCH – CENTER OF EXCELLENCE IN MOLECULAR BIOLOGY, UNIVERSITY OF THE PUNJAB, PAKISTAN

◦ Research project " SNP association studies of Chronic Obstructive Pulmonary disorder in Pakistani population by genotyping"

◦ Cloning, qPCR and DNA quantification, mini sequencing. ◦ Supervisor: Dr. Marium Shahid